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In addition, cholesterol accumulation in MIM interferes with the assembly of respiratory supercomplexes, underlying the impairment in the respiratory capacity, stimulates the generation of superoxide anion resulting in oxidative stress, and stabilizes hypoxia inducible factor-1 (HIF-1), leading to metabolic reprogramming with impaired oxidative phosphorylation and stimulated glycolysis [136]

Cells were placed in an IncuCyte S3 live-cell analysis system (Essen BioScience, Ann Arbor MI, USA) or a CellCyte X Live Cell Analyzer (Cytena, Freiburg im Breisgau, Germany) housed in a humidified incubator containing 95% air and 5% CO 2 at 37 C

It contains compounds that help chelate heavy metals, making it easier for the body to eliminate them

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