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In addition to immune cells, other cell types such as airway epithelial cells may also contribute to trained immunity in allergic disease

doi: 10.1016/j.immuni.2017.01.009 17 PrinzJC

& Hreczycho, G

Following fixation, L3 filets were washed in PTX (PBS and 0.02% Triton X-100) and blocked in PBT (PBS, 0.02% Triton X-100 and 1% NGS) on a nutator and incubated with primary antibodies for either 2 h at room temperature or overnight at 4 C with agitation