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The libraries were confirmed by electrophoresis on a 1% TAE agarose gel and the Agilent BioAnalyzer 2100 after purification by AMPure bead

Blood PD-1+TFh and CTLA-4+CD4+ T cells predict remission after CTLA-4Ig treatment in early rheumatoid arthritis

However, this practice inevitably narrows the clinical spectrum represented in research samples and reduces natural heterogeneity of ASD, limiting the generalizability of findings