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doi: 10.1089/ars.2014.5851 32 Espinosa-DiezCMiguelVMennerichDKietzmannTSnchez-PrezPCadenasSet al

Schoch, H

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For preparation of the membrane fraction the pellet was washed in 5 mL of 50 mM MES-KOH, 20 mM KCl, pH 6.5, centrifuged (150,000 g , 60 min at 4 C), resuspended in 500 L of the same buffer and finally sonicated (20%, 0.5 s 1 , on ice) until membranes were fully suspended