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Coronary heart disease (CHD) is a globally prevalent cardiovascular disorder with a complex pathogenesis associated with multiple risk factors, including high-level low-density lipoprotein cholesterol, smoking, chronic kidney disease, diabetes and hypertension (1)

PMID 34798060

After 10 min at RT, the plates were gently agitated by hand to homogenize the resolubilized crystal violet and OD 550 readings were taken on a Biotek Synergy 2 plate reader