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5 The role of lipid metabolism in comorbidities of depression and other diseases Extensive longitudinal and cross-sectional research has consistently demonstrated a frequent co-occurrence of depression with obesity and metabolic syndrome (181, 182), suggesting shared pathophysiological processes between metabolic disruptions and persistent low-grade metabolic inflammation (181)

This process drives the formation of highly dynamic biomolecular condensatesexemplified by nucleoli, RNA polymerase II condensates, stress granules, and P-bodieswhich enable spatiotemporally precise regulation of biochemical reactions through localized enrichment of reaction components, sequestration of inhibitory factors, or assembly of signaling platforms [12]

According to Rabei et al

Lysates were pre-cleared with 200 L streptavidin magnetic bead slurry (Pierce #88817) with rotation at room temperature for 30 min