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An important aspect in these experiments is the selection from large libraries using recursive cycles of reassembly (optionally in combination with other directed evolution methods described herein) to maximally access all the fortuitous but complex mechanisms that cannot be approached rationally, such as DNA transfer into the cell

Patra JK, Das G, Fraceto LF, Campos EVR, Rodriguez-Torres MDP, Acosta-Torres LS, et al

This phenotype is reminiscent of that observed for pU S 3, which modulates NEC function during viral budding but leads to an accumulation of perinuclear virions in herniations when defective (see introduction)

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