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Freeze-thaw cycles and cold methanol/water were used to extract the intracellular metabolites from the quenched cell pellets according to protocols described by Smart et al

If someone experiences fewer disruptions or a smoother recovery pattern, it may become easier to maintain activity and nutrition routines

Unexplained reductions in the need for phlebotomy should be investigated as occult blood loss may be an underlying cause

Statements Acknowledgments This work has been supported by grants from the Gleis Family Foundation, the University of California, Irvine Multiple Sclerosis Research Center (MSRC) and the National Multiple Sclerosis Foundation