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Binding assay in yeast display format Increasing concentrations of synthetic biotinylated protein target d-Abl SH2 resuspended in Tris-buffered saline (TBS) with 0.1% BSA were incubated with 5 10 5 yeast cells per sample displaying monobodies and a mouse anti-V5 antibody (5 L of 1:300 dilution) in a total volume of 20 L for 30 min at room temperature

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Collectively, these findings might provide a practical framework for standardized hDPC manufacturing and underscore the critical importance of upstream process optimization in the development of cell-based therapies for alopecia