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In the context of transient inflammation, inhibition of MMPs is mediated by TIMPs

Nuclei were pelleted by centrifugation (420 g, 4 C for 5 min) and supernatant was discarded

The warning signs are ping structure of the ear increases suscepti- halos appearing around lights, blurred vision, bility to infection

For the IHC study, after deparaffinization (60 C) and antigen retrieval (1 mM citrate, pH 6, 98 C), slides were treated with 3% H 2 O 2 (5 min) and incubated with primary antibodies (1550 min): cleaved caspase-3 (1:300 dilution, Cell Signaling, 9664S), -H2AX (1:400 dilution, Abcam ab22551), HO-1 (1:400 dilution, Abcam ab52947) and CDA (1:300 dilution, Invitrogen PA5-95616) on a Leica Bond RXm autostainer