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The results demonstrate that GSHee (100 mol/kg, IV) has pronounced positive effects on breathing in freely-moving adult male SpragueDawley rats and that it markedly attenuates the deleterious effects elicited by subsequent injection of fentanyl (75 g/kg, IV) on minute ventilation, A-a gradient and ABG chemistry while extending the analgesic actions of the OR agonist

This allows predicting the drug efficacy in these various patients and cell line backgrounds and estimating the number of patients needed to be treated before seeing a positive outcome (Number Needed to Treat, NNT)

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