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Cell-free plasma hemoglobin and male gender are risk factors for acute kidney injury in low risk children undergoing cardiopulmonary bypass

Simplistic conventional, also inappropriately called 2D, monolayer cultures with a single layer of cells attached to treated polystyrene have unnaturally low cell densities, lack cell-cell interaction or cell-peri and extracellular matrix interactions, and lack the exhibition of the brain tumor nutrient gradients or physiological levels of oxygen (112)

They were then imaged on a fluorescence microscope while being kept vitrified at liquid nitrogen temperatures using a Linkam CMS196 stage

Bhardwaj PV