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Cell-free studies have played a crucial role in the elucidation of biological mechanisms since the late 19th and early 20th century

Mutat Res Rev Mutat Res (2008) 659:1530

The ability to follow transcellular pathways and induce inflammation can vary greatly among meningococcal strains and, therefore, the discrepancies found in the literature between transcellular and paracellular pathways for mechanisms for crossing the nasopharyngeal barrier may be due to the use of different strains in the various studies, as well as different cellular models

Average lifespan has being (hopefully) continuously growing: from ~27 years in the Greco-Roman era, to ~47 years in 1900, and ~77 years by the end of 20 th century [235]