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Measurement: HeLa cells were transfected with plasmids encoding the targeted biosensors

Demedts M, Behr J, Buhl R, et al

Notably, RT-qPCR analysis showed a sharp increase in OCT4 mRNA expression, a marker of pluripotent stem cells and essential for TIC self-renewal [33], in the colonies formed in 3D gels relative to 2D culture conditions (Fig