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Vascular morphometry and immunohistochemical staining analysis The thoracic aortas from the mice were dissected, and 5-m-thick paraffin sections were prepared for vascular morphometric analysis using hematoxylin and eosin staining

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Older age History of a previous thrombotic event Smoking Chronic venous disease Atherosclerosis Abnormal lipid levels Hypertension Type 2 diabetes Obesity Sleep apnea Prolonged inactivity Hospitalization or surgery Infection, transplant, and transfusion reactions Pregnancy Cancer Use of high-dose oral estrogens (eg, hormone replacement therapy or a high-dose birth control pill) How are Blood Clots Treated