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Genetics of Obstructive Sleep Apnea: Vitamin D Receptor Gene Variation Affects Both Vitamin D Serum Concentration and Disease Susceptibility

50100 g were separated by 420% gradient SDS-PAGE blue native gel electrophoresis and either stained for in-gel activities (CI and CIV) or electroblotted on PVDF membrane for immunodetection with specific antibodies against CI (NDUFS3, Abcam, #ab110246) and CII (SDHB, Abcam, #ab14714)

The initial column temperature was 80C, maintained for 0.5 min, then increased to 175C at a rate of 70C/min, and finally to 230C at a rate of 8C/min