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Quantification of RUNX and BMP-2 RUNX (MBS2509507, MyBioSource, San Diego, USA) and BMP-2 (MBS701336, MyBioSource, San Diego, USA) levels were detected in aorta using rat specific enzyme-linked immunosorbent assay (ELISA) kits following the manufactures instruction

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Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications