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[cited 2018 May 10]

coli (1 g) was incubated in the dark with or without NEM (10 mM) in MOPS (50 mM at either pH 7.4 or 10), at room temperature, for 30 min

The unpaired t-tests were either done assuming equal variance (if p-values for F-tests for equal variance were greater than 0.05), or assuming unequal variance (if F-test p-value results were less than 0.05)