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Intracellular carnitine concentrations were calculated from the carnitine concentration determined for the cell pellet extract and the total cell volume of the cell pellet, which was calculated from the final OD 600 of the 2 mL samples at 48 h fermentation and the OD specific total cell volume for starved E

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Since Roche-NGS of a fragment of the NS5B region has been reported to be the ideal method, we have not considered doing a third genotyping technique 23,31,34