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Protein Purification The thawed cell pellet was resuspended in 120 mL of buffer A [50 mM sodium phosphate (NaPi) (pH 7.5), 5% (v/v) glycerol, 200 mM sodium chloride (NaCl)], with 2 mM phenymethylsulfonylfluoride, 2 mM magnesium chloride, lysozyme (30 mg) and DNAse (10 mg) at 4 C, and disrupted by sonication (Branson Ultrasonic)

Toxicity outcomes vary based on particle characteristics (type, size, and surface chemistry), exposure dose and duration, organismal factors (age, sex, species), and co-exposure to other pollutants

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3.5 Modulation of the brain-gut axis Recent investigations have revealed that phytochemicals, in addition to directly targeting the BBB, inhibiting neuroinflammation, and attenuating oxidative stress to improve VCI, can also indirectly protect the BBB by regulating the Microbiota-Gut-Brain Axis (MGBA) and systematically remodeling the central nervous system microenvironment