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For the index PCR, a 25 L reaction containing amplicon PCR product, 0.5 L of Platinum SuperFi II DNA Polymerase, 1 SuperFi II Buffer (Thermo Fisher Scientific), and 0.5 M each of Nextera XT Index Kit v2 index primers (Illumina) was prepared

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Act III, 1994, saw the Republicans mobilizing under Newt Gingrich, defeating Clinton's healthcare reforms, and taking control of the House of Representatives for the first time in forty years