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In macrophage-lineage cells, experimental folate restriction enhances proinflammatory responses, consistent with a model in which limited methyl-donor availability constrains methyltransferase activity and shifts signaling toward activation (114,115)

530 Heat shock protein 27 reduces iron absorption by downregulating TFR1, thus preventing ferroptosis and ameliorating coronary artery disease

The PCR was performed on genomic DNA isolated from individual colonies with the primers flanking the GPX2 gene sequence ( GPX2 _F: 5- TGATGGGGCAAGTAGGAGTC-3, GPX2 _R: 5- CCACACCTGCCCTTTATTGG-3)

These findings position checkpoint ligand dysregulation as a convergence point for DTP plasticity, linking tumor-intrinsic signaling with immune microenvironment reprogramming to sustain therapeutic tolerance