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Formation of biofilms Candida albicans biofilms were formed generally in 96-well black/clear flat bottom polystyrene high binding microplate (Corning Inc.) after seeding the cells (1 10 5 ) in 100 l of RPMI 1640, followed by incubation for 90 min at 37C in an atmosphere of 5% CO 2 and 95% humidity, without shaking to allow cell adhesion

Results: TL was measured for 149 total participants including 103 with HIV mono-infection, 6 with HCV mono-infection, 14 with HIV/HCV co-infection, and 26 uninfected controls

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