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After discarding the supernatant, the proteins on beads were digested by 0.5 g of Trypsin Platinum (mass spec grade, Promega) in 500 l of 25 mM ammonium bicarbonate buffer at 37 C overnight with shaking

This bidirectional behavior highlights a critical context dependence in antioxidant biology, where the same metabolite may either suppress or amplify ferroptotic stress depending on iron availability and intracellular redox flux

But doctors specializing in Environmental Medicine know from their clinical experience that food triggers are highly individual for each patient

The most common extraction methods of chlorogenic acid in Artemisia argyi include decocting, ultrasonic-assisted extraction, cold pressing, subcritical extraction, and supercritical extraction