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Description

6.4 Inhibition of AGEs/RAGE pathway The accumulation of AGEs is a prominent characteristic of diabetes and its complications, particularly DKD

The new supernatant (cytosolic fraction) was directly prepared for SDS-PAGE, and the pellet (membrane fraction) was gently washed with hypo-osmotic buffer and resuspended in GST-lysis buffer supplemented with 0.5% SDS and protease inhibitors