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Single cell data analysis For data analysis, CellBender 92 was used to distinguish cell-containing droplets from empty droplets and DoubletFinder 93 was used to exclude potential droplets

Abbreviations AMPK: AMP-activated protein kinase ATM: Ataxia-telangiectasia mutated ATP: Adenosine triphosphate C/EBP: CCAAT/enhancer-binding protein alpha CKD: Chronic kidney disease CM: Conditioned medium CTGF: Connective tissue growth factor DPP4: Dipeptidyl peptidase 4 EC: Endothelial Cell EMT: Epithelialmesenchymal transition ESCs: Embryonic stem cells ETC: Electron transport chain GBM: Glomerular basement membrane GFR: Glomerular filtration rate GRO-: Growth regulated protein alpha GSK 3B: Glycogen Synthase Kinase 3 Beta hDF: Human dermal fibroblasts HGF: Hepatocyte growth factor HGPS: Hutchitson-Gilford progeria syndrome IGF-1: Insulin-like growth factor-1 JAK: Janus kinase MCP-1: Monocyte chemoattractant protein-1 MMP: Matrix metalloproteinase MQ: Macrophage mTOR: Mammalian target of rapamycin MuSCs: Muscle stem cells NF-B: Nuclear factor kappa-light-chain-enhancer of activated B cells PAI-1: Plasminogen activator inhibitor-1 PD-1: Programmed cell death protein 1 PDGFR-: Platelet-derived growth factor receptor PEC: Parietal Epithelial Cell PGC-1: Peroxisome proliferatoractivated receptor gamma coactivator-1 alpha RAAS: Renin-angiotensin-aldosterone system RBF: Renal blood flow ROS: Reactive oxygen species SASP: Senescence-associated secretory phenotype siRNA: Small interfering RNA SIRT1: Sirtuin-1 SNAI1: Snail family transcriptional repressor 1 TCA: Tricarboxylic acid TEC: Tubular epithelial cells TGF-: Transforming growth factor beta Grhl2: Grainyhead-like 2 TIMP-1: Tissue inhibitor matrix metalloproteinase 1 TNF- : Tumor necrosis factor alpha UUO: Unilateral Ureteral Obstruction -SMA: Smooth muscle actin References Kalache A, et al

Ontgiftingsfunctie: Glutathion speelt een essentile rol in de ontgifting van het lichaam

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