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After overnight incubation, the medium was replaced with Williams E Medium supplemented with primary hepatocyte maintenance supplements (Thermo Fisher Scientific, catalog: CM4000) and incubated for 24 h before compound treatment

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Furthermore, rule-shift learning, a form of cognitive flexibility, was improved by chronic NAC-treatment in the CD2-KO group due to a lower rate of perseverative and random errors, compared to untreated knockouts

Recent studies indicate that Wolbachia may acquire amino acids through the proteasomal activity of its host, a process previously observed in Legionella pneumophila using a mutant deficient in the AnkB F-box effector protein