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This effect is dose-dependent and occurs in both normal and CMT4B3 fibroblasts, indicating that Ser473 modulation by Myo-Ins does not require a fully intact PIP signalling network and occurs upstream of the mitochondrial alterations associated with impaired PIP turnover in CMT4B3 cells

Cureus, 15

In this assay, we analyzed the impact of ENDO series on CpG-induced TLR9 activation using cells expressing the human TLR9 gene upstream of an inducible and secretable embryonic alkaline phosphatase (SEAP) reporter system