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After incubation, the culture medium was collected, centrifuged at 2,000 g for 10 min to remove cellular debris, and the supernatant was carefully harvested for subsequent cytokine quantification using enzyme-linked immunosorbent assays (ELISA) kits: Mouse TNF alpha SimpleStep ELISA Kit (ab208348

By the 28th day, while all female tumor-bearing mice in the other treatment cohorts succumbed, an 80% survival rate was observed in the combined treatment group (Fig