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B12 deficiency on its own leads to memory loss, cognitive decline, depression, constipation, diarrhea, nerve damage / numbness, sore tongue, insomnia, fatigue, abdominal discomfort, easy bruising / anemia, and and memory problems

Two examples are the impairment of clathrin-mediated endocytosis and of nuclear protein degradation, due to polyQ-mediated sequestration of the highly abundant major chaperone heat shock cognate protein 70 (HSC70) and of a low-abundance cochaperone, Sis1p/DNAJB1, respectively [64, 65]

The calcium benefit ended, however, when taking more than 900 mg per day from supplements

Inject subcutaneously, preferably in fatty areas like the stomach