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Tail vein blood (~5-10 l) was collected serially at 0, 30, 60, 90 and 120 min post-challenge through the tail-nick method (19) The blood collection procedure was approved by the Institutional Animal Care and Use Committee of City of Hope in accordance with the NIH Guidelines for Survival Blood Collection in Mice and Rats ( Blood glucose concentration was measured with an Accu-Chek Compact Plus glucometer (Roche Diagnostics Ltd.)

Hexarelin functions primarily by binding to and activating the growth hormone secretagogue receptor (GHS-R) in the brain, similar to its natural counterpart ghrelin [10]

This regimen is considered the best option for inducing ferroptosis for due to reduced cystine, low levels of glutathione or antioxidant, and iron-loaded transferrin is an ideal substrate and condition for ROS generation in cell culture