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Then, total RNA was converted into complementary DNA (cDNA) with iScript Reverse transcriptase Supermix for RT-qPCR (Bio-Rad, CA, United States)

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While standalone selenium supplementation has not yet proven to positively influence the progression of prostate cancer, it has exhibited potential in augmenting the effectiveness of chemotherapy and radiation therapy, as well as in alleviating the side effects that typically accompany these treatments