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B12 is a key factor in the HCG diet, as it helps to break down fats and proteins, aiding your body in burning calories more effectively

for 6 min, following which the reaction was terminated by the addition of a mixture of 10% trichloroacetic acid and 4% methanol, vortexing for 5 s, and centrifuging at 14,000 rpm for 2 min to precipitate the protein