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Description

NAD + is concentrated in the mitochondria, with lower concentrations in the nucleus and cytoplasm 76,77

The neurons were cultured in Neurobasal medium (Gibco) supplemented with 2% B27 (Gibco), 2 mM Glutamax (Gibco) and 50 U/mL penicillin/ streptomycin (Invitrogen) for 2 days in vitro (2DIV) after being treated with specific reagents

HSF cells were cultured in DMEM/F12 medium supplemented with 15% fetal bovine serum (FBS) and 1% penicillinstreptomycin, while macrophages were cultured in specialized complete macrophage medium (PY-H096) at 37C in a 5% CO atmosphere