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In experimental systems, the combination is studied as a model for examining how complementary peptide signaling pathways influence tissue remodeling and cellular repair responses

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doi: 10.1038/nrneph.2016.41 26 OchandoJOrdikhaniFBorosPJordanS

This molecule was duplexed and amplified by 5 cycles of PCR using Phusion HF polymerase (New England Biolabs, NEB), then inserted into blunt II TOPO vector (Invitrogen) to generate a plasmid for electroporation efficiency control (pSSBR)