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To assess that these cells form monolayer on the membrane inserts, transepithelial resistance was routinely monitored using an epithelial volt-ohm-meter including STX-2 electrodes (EVOM, World Precision Instruments, Berlin, Germany)

This mechanical disruption, combined with the medication itself, can trigger localized inflammatory responses including redness, warmth, itching, or mild burning

In contrast, augmented oxidative stress, in turn, stimulates TNF- release 10,17

BSA-SeNPs have been shown to be 7-fold less toxic than selenite in mice, where 113 mg and 14 mg Se/kg body weight were required respectively to reach toxic doses