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Description

Vina J, Gambini J, Lopez-Grueso R, Abdelaziz KM, Jove M, Borras C

For expression and purification of RAG GTPases, HEK293F GnTI- cells were transfected with a total of 1 mg plasmid DNA (550 g for RAGA and 450 g for RAGC) and 4 mg polyethylenimine (PEI) (Sigma-Aldrich) per l at a density of 1.8 10 6 cells per ml