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[6] Chemicals that form DNA adducts [edit] acetaldehyde, a significant constituent of tobacco smoke cisplatin, which binds to DNA and causes crosslinking (leading to cell death) DMBA (7,12-dimethylbenz(a)anthracene) malondialdehyde, a naturally occurring product of lipid peroxidation polycyclic aromatic hydrocarbons (PAHs) [7] nitro-PAHs [8] Nitrosamines [9] Aflatoxins [10] Mustards [11] aromatic amines [12] heterocyclic aromatic amines (HAAs) [13] methylating agents [14] other alkylating agents [15] Haloalkanes [16] Detection methods [edit] 32P-postlabeling assay: 32P-postlabeling assays screen for DNA adducts by transferring 32P-ATP into a carcinogenic labeled nucleotide sequence, with selectivity favoring modified nucleotides

Cross-links were reversed in 5 M NaCl

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