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Previous studies demonstrated zebrafish has two GSDME, GSDMEa and GSDMEb, sharing about 50% sequence similarity to human GSDME, but only GSDMEa could trigger TNF-induced pyroptosis 32

Generally, hydrophilic peptides dissolve well in water or saline, while hydrophobic peptides may require initial dissolution in a small amount of organic solvent (like DMSO or acetic acid) followed by dilution with aqueous buffer