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If a research protocol requires frozen solution storage, the better practice is aliquoting into single-use portions under controlled conditions so each aliquot is thawed once

The combined 10mg vial reconstitutes readily in bacteriostatic water. A common laboratory approach is reconstitution in 2ml to yield a 5mg/ml total working stock (corresponding to 2.5mg/ml CJC-1295 + 2.5mg/ml ipamorelin), or in 5ml for a 2mg/ml total stock (1mg/ml of each component). The bacteriostatic water should be drawn into a sterile syringe and injected slowly down the inside wall of the lyophilised vial, with the peptide cake allowed to dissolve passively rather than through agitation. Reconstituted blend should be aliquoted, refrigerated at 28C and protected from light, with research stocks typically used within 30 days. For dilution maths across alternative volumes, see our reconstitution calculator guide. For long-term storage protocols, see our peptide storage guide

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Benham et al., 2022)