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For measurement, organelle isolation often exaggerates apparent ROS production by disrupting matrix buffering and catalase activity, so in situ readouts with genetically encoded, peroxisome-targeted reporters (e.g., HyPer or roGFP-Orp1 fused to a PTS1/SKL signal) are preferred to capture authentic, compartment-confined dynamics (Fransen and Lismont 2024), Fig

Materials and methods Fish and rearing management Healthy, 80 pathogen-free Nile tilapia fingerlings (15 5) obtained from the National Institute of Oceanography and Fisheries (NIOF), were used in the current study

Due to their sessile nature, plants are required to develop a fast and versatile response system to fluctuating and/or excess light intensity (Scheibe and Dietz 2012