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To eliminate residual red blood cells, the pellet was briefly rinsed with ice-cold Milli-Q water, washed again with buffer, followed by a final centrifugation at 300 g for 10 min

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Importantly, TNF type 1 receptor knockout mice are resistant to the initiation and progression of NAFLD in response to both high-carbohydrate and methionine- and choline-deficient diets 26,27

doi: 10.1038/nature04808 94 TakeuchiYNishikawaH