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Single nucleotide polymorphisms in the human interleukin-1B gene affect transcription according to haplotype context

Mary`s Hospital, College of Medicine, The Catholic University of Korea, Seoul, South Korea 18 Division of Respiratory Medicine, Soon Chun Hyang University Hospital Cheonan, Cheonan, South Korea 19 Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul Metropolitan Government-Seoul National University Boramae Medical Center, Seoul National University College of Medicine, Seoul, South Korea Funding This study was funded by the Boryung Pharmaceuticals

The resulting cDNA was subsequently treated with 1 unit of RNase H (GE Healthcare), diluted with TE buffer, and stored at 20 C until later use

In a targeted DNA methylation study focusing on five genes involved in fibrogenesis modulation, the results showed hypomethylation of pro-fibrogenic genes (TGF1 and PDGF) and hypermethylation of anti-fibrogenic genes (PPAR and PPAR) in advanced MASLD compared to mild cases, suggesting that the methylation status of specific CpGs could potentially predict the severity and progression of MASLD to liver fibrosis [340]