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Tsujimoto S, Kishina M, Koda M, Yamamoto Y, Tanaka K, Harada Y, Yoshida A, Hisatome I

This is one reason that you may not have a reaction to a fresh cooked meal, but you do experience a reaction to the leftovers a couple days later

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ABHD11 purification To purify ABHD11-Flag protein, 1 10 8 HeLa cells expressing ABHD11-Flag were lysed in 1 ml lysis buffer (50 mM TrisHCl pH 7.4, 150 mM NaCl, 1% Triton X-100, 1 Roche complete protease inhibitor cocktail) and rotated at 4 C for 1 h