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Rizzo AN, Haeger SM, Oshima K, Yang Y, Wallbank AM, Jin Y, Lettau M, McCaig LA, Wickersham NE, McNeil JB, Zakharevich I, McMurtry SA, Langout-Astri CJ, Kopf KW, Voelker DR, Hansen KC, Shaver CM, Kerchberger VE, Peterson RA, Kuebler WM, Ochs M, Veldhuizen RA, Smith BJ, Ware LB, Bastarache JA, Schmidt EP

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8) that were uniquely enriched in visit 3 included: (1) ksgA , strA and strB for aminoglycosides, (2) bla CARB-4 , bla CMY-1 , bla CMY-2 , bla CTX-M-1 , bla CTX-M-12 , bla DHA-1 , bla OXA-1 , bla SHV-1 , bla SHV-37 , bla SHV-3 , bla TEM-1A and bla TEM-1B , for beta-lactams, and (3) tetA , tetB , tetC , tetK and tetL for tetracycline

Add three times the volume of protein removal reagent M to the cell pellet and vortex thoroughly