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Alterations in the expression levels of proteins related to fission/fusion processes (such as Opa1, Drp1, MFN1/2, Fis1) 298 and post-translational modifications of Drp1 299,300 may bias mitochondria towards excessive fission, increasing mitochondrial fragmentation, leading to damage in mitochondrial energy biology and accumulation of mitochondrial DNA damage

2015;10(3):e0119479

Finally, riboflavin has also been shown to be under-consumed, with estimates suggesting that 1015% of the global population may be deficient, and over 50% of non-elderly British adults were estimated to have borderline deficiency based on glutathione reductase activation in erythrocytes (Kennedy, 2016)

The membranes were incubated in 5% nonfat skim milk, followed by overnight incubation at 4 C with primary antibodies