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For GSSG determination, the cell extracts were treated with 4-vinyl pyridine (Sigma-Aldrich) for GSH derivatization, and the excess of 4-vinyl pyridine was neutralized with triethanolamine

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W J Gastroenterol 21:993996 Thirumoorthy N, Shyam Sunder A, Manisenthil Kumar KT, Senthil kumar M, Ganesh Malay Chatterjee GNK (2011) A review of metallothionein isoforms and their role in pathophysiology