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2 Materials and methods 2.1 Microorganisms, culture media and conditions Levilactobacillus brevis CRL 2013, belonging to the CERELA culture collection, was routinely grown in modified MRS broth (mMRS, Laboratorio Britania, Buenos Aires, Argentina) containing 10 g/L fructose and 10 g/L glucose (MRS-GF) as carbohydrates at 30C for 16 h

Linear regression and linear mixed model analyses were conducted on scaled and log1p-normalised metabolites or metabolite ratios to further inspect variables with significant differences between groups

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