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Combining these peptides allows researchers to investigate complementary GH-stimulating pathways and receptor signaling synergy

Experimental application must remain within controlled, in vitro environments

Ligand binding drives receptor autophosphorylation, IRS-1/IRS-2 adapter recruitment, and activation of two primary downstream cascades: PI3K/Akt/mTOR (governing protein synthesis, glucose uptake, and cell survival) and MAPK/ERK (regulating cell proliferation and differentiation)

For immunohistochemistry, primary antibodies and the respective fluorophore-linked secondary ones were diluted 1:500 in the blocking buffer