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Besides the presently reported decreased PST activities, blood hyperserotonemia was also reported to result from increased SERT activity due: (i) to the rare Ala56 ASD-associated gain-of-function mutation in the SLC6A4 gene found in some ASD patients 13 which altered the communication and social domains in knock-in mice 85 or (ii) to a variant in the promoter of the ITGB3 gene 86 which encodes the -chain of the platelet integrin IIb 3 known to interact physically with SERT to promote its externalization and transport activity 87

From allicin, several different organosulfur compounds are produced

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Then, the trypsin/EDTA solution was neutralized with Dulbeccos modified Eagles medium (DMEM, #22320-022, ThermoFisher Scientific, Waltham, MA, USA), supplemented with 10% (v/v) fetal bovine serum (FBS, #S181C, Biowest, Nuaill, France)